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KMID : 0368420070500050573
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Journal of Plant Biology
2007 Volume.50 No. 5 p.573 ~ p.576
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Biomass production ofAnoectochilus formosanus hayata in a bioreactor system
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Yoon Yeo-Joong
Murthy Hosakatte Niranjana
Hahn Eun-Joo
Paek Kee-Yoeup
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Abstract
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We investigated the factors that affect biomass production fromAnoectochilus formosanus in a bioreactor system. Those factors included inoculum size, initial sucrose concentration, media supplements, photosynthetic photon flux density (PPFD), and cuIturing methods. An inoculum size of 8 g L?1 was most suitable for shoot proliferation; biomass accumulation was optimized when the medium was supplemented with 3% sucrose compared with sucrose-free media or those containing concentrations of 6% or 9%. This accumulation also was enhanced under a PPFD of 50 μmol m2 s?1. Likewise, the addition of coconut water (50 mL L?1) plus activated charcoal (0.5 mg L?1) to our Hyponex medium proved most beneficial. Comparative studies among three bioreactor systems ? continuous immersion, raft (net), and temporary immersion (the ebb and flood system) ? revealed that shoot proliferation and biomass accumulation were more efficient when culturing was performed under continuous immersion.
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KEYWORD
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Anoechtochilus formosanus, bioreactor culture, jewel orchid
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